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ROS Quantification by Spin-Trapping and EPR Spectroscopy Cells (5 × 10 6 /0.1 ml) were stimulated for 2 h with LPS, then incubated with 50 mM 5-tert-butoxycarbonyl-5-methyl-1-pyrroline-N-oxide (BMPO), synthetized as described previously (16), in PBS containing 1 mM diethylenetriaminepentaacetic acid

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This IGF-1 release completes the regulatory loop by providing the negative feedback signal to the hypothalamus shown in Section 3