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SASP expression was assessed via immunofluorescence in cocultured cells by washing with PBS, fixing with 4% paraformaldehyde, permeabilizing with 0.1% Triton X-100 in PBS, blocking with 5% bovine serum albumin, and incubating with anti-TNFα (80258-6-RR, Proteintech, USA) or IL-1β (ab315084, Abcam, UK) primary antibodies overnight at 4 °C