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Hst3p, a key sirtuin histone deacetylase, mediates H3K56 deacetylation to maintain chromatin repression, and its inhibition leads to genome-wide H3K56ac enrichment, which further dysregulates the transcription of virulence-associated genes including adhesins, phenotypic switching regulators and hyphal formation-related factors, thereby disrupting yeast-hyphae transition, white-opaque switching and host adhesion processes essential for C

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H2DCFDA fluorescence (FL-1) in propidium iodide-negative cells (FL-3) was tested by a FACSCalibur Flow Cytometer